Manire, Charles A.

5 publications

Colbert, Debborah E.; Fellner, Wendi; Bauer, Gordon Bruce; Manire, Charles A.; Rhinehart, Howard L. (detail)
2001Husbandry and research training of two Florida manatees (Trichechus manatus latirostris).
Aquatic Mammals 27(1): 16-23.
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Manire, Charles A.; Walsh, Catherine J.; Rhinehart, Howard L.; Colbert, Debborah E.; Noyes, David R.; Luer, Carl A. (detail)
2003Alterations in blood and urine parameters in two Florida manatees (Trichechus manatus latirostris) from simulated conditions of release following rehabilitation.
Zoo Biology 22(2): 103-120.
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Walsh, Catherine J.; Stuckey, J. E.; Cox, H.; Smith, B.; Funke, C.; Stott, Jeffrey L.; Colle, C.; Gaspard, J.; Manire, Charles A. (detail)
2007Production of nitric oxide by peripheral blood mononuclear cells from the Florida manatee, Trichechus manatus latirostris.
Veter. Immunol. Immunopathol. 118(3-4): 199-209.
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Tripp, Kathleen M.; Verstegen, John P.; Deutsch, Charles J.; Bonde, Robert K.; De Wit, Martine; Manire, Charles A.; Gaspard, Joseph; Harr, Kendal E. (detail)
2010Evaluation of adrenocortical function in Florida manatees (Trichechus manatus latirostris).
Zoo Biology 29: 1-15. 3 tabs.
—[Or: vol. 30(1): 17-31, 2011?]
Grant, Rebecca J.; Kelley, Karen L.; Maruniak, James E.; Garcia-Maruniak; Alejandra; Barrett, Tom; Manire, Charles A.; Romero, Carlos H. (detail)
2010Expression from baculovirus and serological reactivity of the nucleocapsid protein of dolphin morbillivirus.
Veterinary Microbiology 143(2-4): 384-388. 1 tab. 2 figs. DOI: 10.1016/j.vetmic.2009.11.019. July 14, 2010.
—ABSTRACT: The nucleocapsid (N) protein of dolphin morbillivirus (DMV) was expressed from a baculovirus (Autographa californica nuclear polyhedrosis virus) vector and shown by SDS-PAGE and Western blot analysis to be about 57 kDa. Transmission electron microscopy revealed fully assembled nucleocapsid-like particles (NLPs) exhibiting the typical helical herringbone morphology. These NLPs were approximately 20-22 nm in diameter and varied in length from 50 to 100 nm. Purified DMV-N protein was used as antigen in an indirect ELISA (iELISA) and shown to react with rabbit and human antisera to measles virus (MV) and dog sera with antibodies to canine distemper virus (CDV). The iELISA was used for the demonstration of morbillivirus antibodies in the serum of cetaceans and manatees, showing potential as a serological tool for the mass screening of morbillivirus antibodies in marine mammals.